2QV3
Crystal Structure of the Helicobacter pylori Vacuolating Toxin p55 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-12 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.606, 56.606, 256.895 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.030 - 2.401 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.171 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.410 |
| Number of reflections | 19101 | |
| <I/σ(I)> | 14.7 | 4.7 |
| Completeness [%] | 96.7 | 96.7 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 18-23% PEG 1500, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
