2QV1
Crystal structure of HCV NS3-4A V36M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-02 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 228.760, 228.760, 75.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.760 - 2.400 |
Rwork | 0.228 |
R-free | 0.26500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | HCV NS3-4A wild type protease |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNX (2005) |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.052 | 0.052 |
Number of reflections | 26385 | |
<I/σ(I)> | 16.5 | 16.5 |
Completeness [%] | 91.2 | 91.2 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 1.4M NaCl, 0.3M KH2PO4, 0.1M MES,10.0mM PME, protein concentration 8mg/ml, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |