2QV0
Crystal structure of the response regulatory domain of protein mrkE from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 61 |
| Unit cell lengths | 56.239, 56.239, 188.037 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.28200 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.594 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.826 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.105 | 0.681 |
| Number of reflections | 13141 | |
| <I/σ(I)> | 20.5 | 3.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 11.5 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 100mM Tris-HCl pH 8.5, 1.26M Ammonium sulfate, 200mM Lithium sulfate, VAPOR DIFFUSION, temperature 294K |
