2QUY
Truncated mutant ASN175ALA of penicillin v acylase from bacillus sphaericus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-13 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.286, 379.383, 102.013 |
| Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.17684 |
| Rwork | 0.175 |
| R-free | 0.20293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.676 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 370615 |
| Completeness [%] | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | pH 6.4, VAPOR DIFFUSION, HANGING DROP |
