2QUQ

Crystal Structure of the Essential Inner Kinetochore Protein Cep3p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07182
Spacegroup name	P 43 21 2
Unit cell lengths	84.629, 84.629, 230.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.800
Rwork	0.228
R-free	0.28500
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.336
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	20365
<I/σ(I)>	25.43	1.98
Completeness [%]	94.2	67.5
Redundancy	6.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM HEPES, 5% PEG 4000, 500 mM NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM HEPES, 5% PEG 4000, 500 mM NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM HEPES, 5% PEG 4000, 500 mM NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 298K