2QUA
Crystal structure of LipA from Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000, 0.780 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 115.070, 115.070, 104.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.270 - 1.950 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.093 | 0.730 |
| Number of reflections | 51634 | |
| <I/σ(I)> | 12.8 | 1.1 |
| Completeness [%] | 94.5 | 73 |
| Redundancy | 4.88 | 4.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 281 | 26% MPD, 0.1 M Na-Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 281K, pH 4.60 |
