2QU8

Crystal structure of putative nucleolar GTP-binding protein 1 PFF0625w from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97937
Spacegroup name	P 21 21 2
Unit cell lengths	56.377, 77.162, 40.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.720 - 2.010
R-factor	0.225
Rwork	0.223
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.187
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.076	0.441
Number of reflections	12175
<I/σ(I)>	11.9	3.25
Completeness [%]	100.0	100
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	28% PEG 2000 MME, 0.1 M Bis-Tris pH 6.5, 1 mM GDP, 2 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	28% PEG 2000 MME, 0.1 M Bis-Tris pH 6.5, 1 mM GDP, 2 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	28% PEG 2000 MME, 0.1 M Bis-Tris pH 6.5, 1 mM GDP, 2 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K