2QU0
Crystal structure determination of sheep methemoglobin at 2.7 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.231, 70.695, 131.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.890 - 2.700 |
| R-factor | 0.201 |
| Rwork | 0.195 |
| R-free | 0.25441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g08 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.485 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.135 | |
| Number of reflections | 15460 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 95.7 | 97 |
| Redundancy | 3.1 | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, Unbuffered condition, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
