2QTY
Crystal Structure of mouse ADP-ribosylhydrolase 3 (mARH3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.877, 60.096, 91.782 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.16794 |
| Rwork | 0.167 |
| R-free | 0.19761 |
| Starting model (for MR) | 2foz |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.457 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.800 |
| Number of reflections | 51153 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 93.8 | 89.2 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | PEG 4000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
