2QTM
Crystal Structure of Nicotinate Mononucleotide Adenylyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 |
Unit cell lengths | 97.026, 97.026, 85.095 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.217 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kaq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.474 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.085 | 0.504 |
Number of reflections | 16464 | |
<I/σ(I)> | 11.1 | |
Completeness [%] | 91.9 | 69.3 |
Redundancy | 5.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | sodium chloride, cobalt chloride, tascimate, glycerol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |