2QTH
Crystal structure of a GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.991, 72.403, 96.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.300 - 2.000 |
| R-factor | 0.22593 |
| Rwork | 0.224 |
| R-free | 0.26394 |
| Structure solution method | SAD |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.127 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.388 |
| Number of reflections | 30737 | |
| <I/σ(I)> | 13.9 | 3.6 |
| Completeness [%] | 97.3 | 96.7 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.05M Cadmium sulfate, 0.1M HEPES, 0.8M Sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
