2QTE
Crystal Structure of Novel Immune-Type Receptor 11 Extracellular Fragment Mutant N30D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.476, 68.662, 99.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.200 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.059 | 0.160 |
| Number of reflections | 32478 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 92.0 | 91.9 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG 8000, 0.2M Calcium Acetate hydrate, 0.1M Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
