2QT8
Coproporphyrinogen III oxidase from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.083, 53.808, 65.828 |
| Unit cell angles | 86.28, 77.28, 60.99 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19300 |
| Starting model (for MR) | 1vju |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.027 |
| Data reduction software | TRUNCATE (CCP4_5.99) |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.980 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.049 | 0.034 | 0.229 |
| Number of reflections | 58682 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 94.6 | 99.3 | 77 |
| Redundancy | 3.3 | 4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 1 ul Protein 11 mg/ml, 1 ul Crystallization buffer: 35% PEG 5000 MME, 200 mM Ammonium sulfate, 100 mM MES pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
