2QT6
Crystal Structure Determination of a Blue Laccase from Lentinus Tigrinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.218, 111.613, 97.086 |
| Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.15 |
| Rwork | 0.150 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.641 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.483 | |
| Number of reflections | 180651 | |
| <I/σ(I)> | 20.3 | 3.3 |
| Completeness [%] | 99.1 | 97.2 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 22% PEG 4000, 0.2 M CaCl2, 100 mM TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
