2QT5
Crystal Structure of GRIP1 PDZ12 in Complex with the Fras1 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.100, 75.925, 126.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.150 - 2.300 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.157 | 37.960 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.060 | 0.024 | 0.355 |
| Total number of observations | 6567 | 20674 | |
| Number of reflections | 25986 | ||
| <I/σ(I)> | 11.1 | 9.9 | 2.2 |
| Completeness [%] | 99.9 | 98.6 | 99.8 |
| Redundancy | 8.2 | 7.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M magnesium acetate, 0.1M sodium cacodylate pH 6.5, 20% PEG 8K, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
