2QSU
Structure of Arabidopsis thaliana 5'-Methylthioadenosine nucleosidase in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.250, 126.910, 45.340 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 63.500 - 2.000 |
| R-factor | 0.20341 |
| Rwork | 0.198 |
| R-free | 0.25377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Formycin-A complexed structure of A. thaliana MTAN |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.498 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.081 | 0.262 |
| Number of reflections | 28189 | |
| <I/σ(I)> | 8.9 | 4 |
| Completeness [%] | 94.6 | 90.7 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | NH4Cl, PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
