2QST
Crystal structure of the V39C mutant of the N-terminal domain of carcinoembryonic antigen (CEA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 127.228, 127.228, 166.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.890 - 2.900 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25000 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.458 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.228 | |
| Number of reflections | 11745 | |
| <I/σ(I)> | 9 | 1.2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 8.9 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 2.5 M NaCl, 0.1 M Tris-HCl, pH 7.0, vapor diffusion, temperature 277K |
