2QSR

Crystal structure of C-terminal domain of transcription-repair coupling factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-09
Detector	MAR CCD 165 mm
Wavelength(s)	0.97930
Spacegroup name	P 61 2 2
Unit cell lengths	84.891, 84.891, 122.021
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.450 - 3.100
R-factor	0.187
Rwork	0.184
R-free	0.24300
RMSD bond length	0.008
RMSD bond angle	1.116
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.127	0.817
Number of reflections	5156
<I/σ(I)>	4.6	3.24
Completeness [%]	100.0	100
Redundancy	19.4	19.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	1.6 M Sodium citrate pH 6.5, temperature 298K, VAPOR DIFFUSION, SITTING DROP