2QSQ
Crystal structure of the N-terminal domain of carcinoembryonic antigen (CEA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 |
| Unit cell lengths | 132.531, 132.531, 82.762 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.950 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20300 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.802 |
| Number of reflections | 38912 | |
| <I/σ(I)> | 15 | 1 |
| Completeness [%] | 97.8 | 83.4 |
| Redundancy | 4.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 3.0 M NaCl, 100 mM Tris-HCl pH 7.0, temperature 277K, VAPOR DIFFUSION |
