2QSH
Crystal structure of Rad4-Rad23 bound to a mismatch DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.644, 79.644, 403.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.805 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 31854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 50 mM bis-tris propane, 100 mM sodium chloride, 15% (v/v) isopropanol, 10 mM calcium chloride and 5 mM dithiothreitol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | bis-tris propane | ||
| 10 | 1 | 2 | dithiothreitol | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | isopropanol | ||
| 5 | 1 | 1 | dithiothreitol | ||
| 6 | 1 | 2 | bis-tris propane | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | CaCl2 | ||
| 9 | 1 | 2 | isopropanol |
