2QSF

Crystal structure of the Rad4-Rad23 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97922
Spacegroup name	C 1 2 1
Unit cell lengths	83.167, 115.711, 113.947
Unit cell angles	90.00, 105.70, 90.00

Refinement procedure

Resolution	15.000 - 2.350
Rwork	0.226
R-free	0.24500
RMSD bond length	0.006
RMSD bond angle	1.192
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.350
Number of reflections	41336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100 mM sodium phosphate, 8% (w/v) PEG 2000, 2 mM dithiothreitol, pH 6.5, hanging-drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP