2QSD
Crystal structure of a protein Il1583 from Idiomarina loihiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-24 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 185.401, 185.401, 185.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.21632 |
| Rwork | 0.215 |
| R-free | 0.25318 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.720 |
| Number of reflections | 142014 | |
| <I/σ(I)> | 6.5 | 1.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 2M Ammonium sulfate, 0.1M Tris-HCl, 10% Glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
