2QSB
Crystal structure of a protein from uncharacterized family UPF0147 from Thermoplasma acidophilum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931, 0.97949 |
| Spacegroup name | P 65 |
| Unit cell lengths | 50.584, 50.584, 59.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.550 - 1.300 |
| R-factor | 0.149 |
| Rwork | 0.147 |
| R-free | 0.17800 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.099 | 0.370 |
| Number of reflections | 23474 | |
| <I/σ(I)> | 9.4 | 3.5 |
| Completeness [%] | 98.2 | 97.3 |
| Redundancy | 11.1 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2M Ammonium sulfate, 0.1M Tri-HCl pH 7.0, 0.2M Lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
