2QS9
Crystal structure of the human retinoblastoma-binding protein 9 (RBBP-9). NESG target HR2978
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.081, 130.340, 39.047 |
| Unit cell angles | 90.00, 115.87, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.720 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (D/E) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.046 | 0.082 |
| Number of reflections | 67796 | |
| <I/σ(I)> | 25.9 | 14.4 |
| Completeness [%] | 96.7 | 85.5 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 5 | 291 | 40% PEG 4000, 0.1M Magnesium nitrate, 0.1M Sodium acetate pH 5.0, MICROBATCH UNDER OIL, temperature 291K |
