2QRR
Crystal structure of the soluble domain of the ABC transporter, ATP-binding protein from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.134, 47.780, 53.347 |
| Unit cell angles | 90.00, 118.48, 90.00 |
Refinement procedure
| Resolution | 46.880 - 1.710 |
| R-factor | 0.203 |
| Rwork | 0.199 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.680 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 23458 | |
| <I/σ(I)> | 10.9 | 2.2 |
| Completeness [%] | 95.7 | 89.7 |
| Redundancy | 4.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 2.4 M Ammonium phosphate dibasic, 0.1 M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
