2QRK

Crystal Structure of AMP-bound Saccharopine Dehydrogenase (L-Lys Forming) from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-03-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	45.970, 69.106, 127.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.750
R-factor	0.2
Rwork	0.199
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2Q99 (BACKBONE ONLY)
RMSD bond length	0.015
RMSD bond angle	1.412
Data reduction software	d*TREK
Data scaling software	d*TREK (9.6D)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.010	29.010	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.034	0.020	0.154
Total number of observations		26782	19314
Number of reflections	41009
<I/σ(I)>	25.9	63.4	8.8
Completeness [%]	97.8	98.6	91.9
Redundancy	5.87	5.96	5.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG-MME 2000, Bis-Tris, AMP, pH 6.5, vapor diffusion, hanging drop, temperature 277K