2QRJ

Crystal Structure of Sulfate-bound Saccharopine Dehydrogenase (L-Lys Forming) from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-11-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	112.628, 55.206, 75.039
Unit cell angles	90.00, 116.24, 90.00

Refinement procedure

Resolution	30.000 - 1.600
R-factor	0.223
Rwork	0.221
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2Q99 (BACKBONE ONLY)
RMSD bond length	0.016
RMSD bond angle	1.474
Data reduction software	d*TREK
Data scaling software	d*TREK (9.6D)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.750	28.750	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.060	0.031	0.477
Total number of observations		40780	28176
Number of reflections	53059
<I/σ(I)>	11.3	37.4	3
Completeness [%]	97.1	99.5	92.9
Redundancy	6.71	7.2	5.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG-MME 2000, Tris, (NH4)2SO4, pH 7.0, vapor diffusion, hanging drop, temperature 277K