2QRD
Crystal Structure of the Adenylate Sensor from AMP-activated Protein Kinase in complex with ADP and ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.263, 78.365, 109.014 |
| Unit cell angles | 90.00, 124.18, 90.00 |
Refinement procedure
| Resolution | 35.940 - 2.410 |
| R-factor | 0.19171 |
| Rwork | 0.188 |
| R-free | 0.26155 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2ooy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.748 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.517 |
| Number of reflections | 45186 | |
| <I/σ(I)> | 19.7 | 3.5 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 6-10% PEG 3350, 0.1M Sodium Citrate, pH 5.5, 5mM ADP, 5mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
