2QR8
2.0A X-ray structure of C-terminal kinase domain of p90 ribosomal S6 kinase 2 (RSK2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2006-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.912, 46.912, 291.076 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21755 |
| Rwork | 0.215 |
| R-free | 0.25886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qr7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 23175 | |
| <I/σ(I)> | 30.6 | |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 7.5%-10% PEG3350, 50mM Ammonium sulfate, 0.1M Hepes , VAPOR DIFFUSION, SITTING DROP |
