2QR6

Crystal structure of IMP dehydrogenase/GMP reductase-like protein (NP_599840.1) from Corynebacterium glutamicum ATCC 13032 Kitasato at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97925, 0.97904
Spacegroup name	I 4
Unit cell lengths	127.130, 127.130, 54.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.656 - 1.500
R-factor	0.17
Rwork	0.169
R-free	0.18800
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.751
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.656	29.656	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.036	0.019	0.605
Number of reflections	69856	13294	12226
<I/σ(I)>	10.61	34.2	1.4
Completeness [%]	97.6	96.4	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 0.2M Mg(OAc)2, 30.0% MPD, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K