2QR5
Aeropyrum pernix acylaminoacyl peptidase, H367A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-20 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.270, 105.040, 114.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.200 |
| R-factor | 0.17959 |
| Rwork | 0.177 |
| R-free | 0.22225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1VE6 (ONE MONOMER) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.118 | 0.467 |
| Number of reflections | 58729 | |
| <I/σ(I)> | 11.5 | 3.61 |
| Completeness [%] | 93.2 | 93.6 |
| Redundancy | 3.71 | 3.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.35 | 293 | 169 mM sodium acetate, 0.4 mM EDTA, 2% PEG 4000 0.51% beta-octyl-glucoside, pH 6.35, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
