2QR3
Crystal structure of the N-terminal signal receiver domain of two-component system response regulator from Bacteroides fragilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.044, 56.044, 73.622 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23400 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.535 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | 0.433 |
| Total number of observations | 13174 | |
| Number of reflections | 12743 | |
| <I/σ(I)> | 19 | 4.2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 2.8M Sodium acetate, pH 7.0, VAPOR DIFFUSION, temperature 294K |
