2QQW
Crystal structure of a cell-wall invertase (D23A) from Arabidopsis thaliana in complex with sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 32 |
| Unit cell lengths | 105.877, 105.877, 50.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.570 - 2.800 |
| R-factor | 0.19153 |
| Rwork | 0.188 |
| R-free | 0.25231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ac1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.333 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 12825 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M sodium cacodylate, 0.1M zinc acetate, 22.5% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
