2QQM
Crystal Structure of the a2b1b2 Domains from Human Neuropilin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.177, 68.178, 66.613 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2QQI (b1b2 domains) 2QQK (a2 domain) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.455 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.053 | ||
| Number of reflections | 30901 | ||
| <I/σ(I)> | 13.8 | 3.2 | |
| Completeness [%] | 97.5 | 99.3 | 88.7 |
| Redundancy | 3.5 | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
