2QQ5
Crystal structure of human SDR family member 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-05 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.225, 87.854, 100.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.210 - 1.800 |
| R-factor | 0.15881 |
| Rwork | 0.157 |
| R-free | 0.19719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2AE1 1ae1 2ew8 1zem 1ahh 1vl8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.213 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.210 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.567 |
| Number of reflections | 91499 | |
| <I/σ(I)> | 17.8 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.4M Na Malonate pH 7.0, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
