2QPX

Crystal structure of putative metal-dependent hydrolase (YP_805737.1) from Lactobacillus casei ATCC 334 at 1.40 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97917, 0.97891
Spacegroup name	I 2 3
Unit cell lengths	142.170, 142.170, 142.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.025 - 1.400
R-factor	0.135
Rwork	0.134
R-free	0.16200
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.543
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.025	29.025	1.450
High resolution limit [Å]	1.400	3.010	1.400
Rmerge	0.067	0.011	0.011
Number of reflections	93379	18383	18055
<I/σ(I)>	16.16	59.9	1.7
Completeness [%]	99.8	99.7	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	NANODROP, 1.0M LiCl, 20.0% PEG 6000, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K