2QPN
GES-1 beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.410, 80.820, 71.440 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.100 |
| Rwork | 0.133 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.046 | 0.349 |
| Number of reflections | 163829 | |
| <I/σ(I)> | 11.47 | 2.69 |
| Completeness [%] | 93.4 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 10% PEG8000, 10% PEG1000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
