2QPM
Leu492Ala mutant of Maize cytokinin oxidase/dehydrogenase complexed with benzylurea inhibitor CPBU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 248.500, 49.700, 50.500 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.850 |
| Rwork | 0.221 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w1o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 50659 | |
| Completeness [%] | 97.6 | 98.5 |
| Redundancy | 4.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 31% (W/v) PEG 5000, 0.5% (w/v)n-octyl beta-D-glucoside, 0.1 M Tris-HCl , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
