2QP2

Structure of a MACPF/perforin-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-02-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	97.439, 154.507, 43.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.210 - 2.000
R-factor	0.182
Rwork	0.180
R-free	0.20400
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	0.961
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.047	0.426
Number of reflections	44074
<I/σ(I)>	15.8	2.1
Completeness [%]	96.9	79.1
Redundancy	3.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	20% PEG8000, 0.1M MES pH6.0, 0.2M Calcium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6	298	20% PEG8000, 0.1M MES pH6.0, 0.2M Calcium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K