2QO9

Human EphA3 kinase and juxtamembrane region, phosphorylated, AMP-PNP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2006-07-11
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1 21 1
Unit cell lengths	53.839, 38.205, 76.474
Unit cell angles	90.00, 102.16, 90.00

Refinement procedure

Resolution	18.510 - 1.550
R-factor	0.183
Rwork	0.182
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsf
RMSD bond length	0.012
RMSD bond angle	1.375
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.630
High resolution limit [Å]	1.550	4.330	1.550
Rmerge	0.038	0.027	0.134
Number of reflections	42955
<I/σ(I)>	36.4
Completeness [%]	98.4	97.5	92.2
Redundancy	5.9	5.9	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 mg/mL Protein, 25% PEG 3350, 0.2M Ammonium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K