2QNW
Toxoplasma gondii apicoplast-targeted acyl carrier protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 38.814, 38.814, 104.617 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.880 - 1.900 |
| R-factor | 0.17449 |
| Rwork | 0.172 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fae |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.546 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 7707 | |
| <I/σ(I)> | 37.15 | 5.5 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 9.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 30% PEG 1500, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
