2QNG
Crystal structure of unknown function protein SAV2460
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-26 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.231, 40.701, 49.436 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 49.090 - 1.400 |
| R-factor | 0.16633 |
| Rwork | 0.165 |
| R-free | 0.18560 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.061 | 0.353 |
| Number of reflections | 33631 | |
| <I/σ(I)> | 31.99 | 2.96 |
| Completeness [%] | 98.9 | 93.2 |
| Redundancy | 6.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-HCl pH 8.5, 0.2M CaCl2, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
