2QN9

Glycogen Phosphorylase in complex with N-4-aminobenzoyl-N'-beta-D-glucopyranosyl urea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	293
Detector technology	CCD
Collection date	2002-10-31
Detector	MAR CCD 165 mm
Wavelength(s)	0.9076
Spacegroup name	P 43 21 2
Unit cell lengths	128.318, 128.318, 116.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.187
R-free	0.20510
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	65665
<I/σ(I)>	16.2	2.5
Completeness [%]	99.4	92.9
Redundancy	5.6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM Bes, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K