2QMO
Crystal structure of dethiobiotin synthetase (bioD) from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.095, 37.454, 69.636 |
| Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.470 |
| R-factor | 0.15 |
| Rwork | 0.148 |
| R-free | 0.17900 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.069 | 0.444 |
| Number of reflections | 35286 | |
| <I/σ(I)> | 48.754 | 3.1 |
| Completeness [%] | 99.0 | 91.3 |
| Redundancy | 7.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 273 | VAPOR DIFFUSION, temperature 273K |
