2QMB
Structure determination of haemoglobin from Turkey(meleagris gallopavo) at 2.8 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.000, 79.795, 103.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.430 - 2.800 |
| R-factor | 0.18029 |
| Rwork | 0.172 |
| R-free | 0.28356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.041 |
| RMSD bond angle | 3.343 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.176 | |
| Number of reflections | 13851 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 98.7 | 99.3 |
| Redundancy | 4.18 | 3.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10mM Tris-HCl, 35% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
