2QMA
Crystal structure of glutamate decarboxylase domain of diaminobutyrate-pyruvate transaminase and L-2,4-diaminobutyrate decarboxylase from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.431, 122.776, 64.289 |
| Unit cell angles | 90.00, 95.74, 90.00 |
Refinement procedure
| Resolution | 37.670 - 1.810 |
| R-factor | 0.1827 |
| Rwork | 0.181 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.091 | 0.538 |
| Number of reflections | 86431 | |
| <I/σ(I)> | 31.4 | 1.99 |
| Completeness [%] | 95.9 | 70.8 |
| Redundancy | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M Lithium sulfate, 0.1 M Bis-Tris buffer, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
