2QM7
MeaB, A Bacterial Homolog of MMAA, Bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.060, 79.150, 71.070 |
| Unit cell angles | 90.00, 110.93, 90.00 |
Refinement procedure
| Resolution | 29.920 - 1.850 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.24700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Initial model derived from Se-Met derivative solved by MAD. |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.495 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 55277 | |
| <I/σ(I)> | 27 | 10.5 |
| Completeness [%] | 98.5 | 89.6 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris, pH 5.5, 0.2 M Li2SO4, and 17% PEG 3350., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
