2QM1
Crystal structure of glucokinase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.083, 131.260, 82.763 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 39.430 - 2.020 |
| R-factor | 0.19 |
| Rwork | 0.186 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.693 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 86418 | |
| <I/σ(I)> | 7.9 | 2.32 |
| Completeness [%] | 99.4 | 94.6 |
| Redundancy | 7.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 295 | 0.2M NaCl, 0.1M Bis-Tris pH 5.7, 22.5% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
