2QM0
Crystal structure of BES protein from Bacillus cereus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930, 0.97950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.849, 135.058, 200.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.940 - 1.840 |
| R-factor | 0.18879 |
| Rwork | 0.184 |
| R-free | 0.22828 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.940 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 55348 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 95.5 | 65.8 |
| Redundancy | 8.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 2.0 M Ammonium sulfate, 0.1 M Sodium cacodylate pH 6.5, 0.2 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
