2QLZ
Crystal Structure of Transcription Factor PF0095 from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.132, 174.699, 52.249 |
| Unit cell angles | 90.00, 93.11, 90.00 |
Refinement procedure
| Resolution | 19.430 - 2.500 |
| R-factor | 0.22791 |
| Rwork | 0.226 |
| R-free | 0.27132 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.430 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 28832 | |
| <I/σ(I)> | 43.6 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.4 | 291 | 20mM Calcium Chloride, 25% MPD, pH 4.4, modified microbatch, temperature 291K |
